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Ocean Optics HR2000CG-UV-NIR User Manual

Page 25

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Sample Experiments

HR2000 High-Resolution Fiber Optic Spectrometer

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3. Place a sample of the solvent into a cuvette and take a reference spectrum. You must take a reference

spectrum before measuring absorbance.

Note: Do not put the sample itself in the path when taking a reference spectrum, only the solvent.

Click the Store Reference spectrum icon on the toolbar or select Spectrum | Store Reference from the
menu bar to store the reference. This command merely stores a reference spectrum in memory. You
must select File | Save | Reference from the menu bar to permanently save the spectrum to disk.

4. Block the light path to the spectrometer. Then, take a dark spectrum by clicking the Store Dark

Spectrum icon on the toolbar or by selecting Spectrum | Store Dark from the menu bar. This command
merely stores a dark spectrum in memory. You must select File | Save | Dark from the menu to
permanently save the spectrum to disk.

Note: If possible, do not turn off the light source when taking a dark spectrum. If you must turn off your

light source to store a dark spectrum, allow enough time for the lamp to warm up again before
continuing your experiment. After the lamp warms up again, store a new reference (Step 3).

You must take a dark spectrum before measuring absorbance.

5. Put the sample in place and ensure that the light path is clear. Then, take an absorbance measurement

by clicking on the Absorbance Mode icon on the toolbar or selecting Spectrum | Absorbance Mode
from the menu. To permanently save the spectrum to disk, click the Save icon on the toolbar or select
File | Save | Processed from the menu bar.

Note: If you change any sampling variable (integration time, averaging, smoothing, angle, temperature,

fiber size, etc.), you must store a new dark and reference spectrum.