Hoefer SP-2001 Vision Life Spectrophotometer - Manual (Page 5)

Version 1.0
Page 5
INTRODUCTION
Your spectrophotometer
Your spectrophotometer is a simple-to-use UV/Visible instrument with a CCD array detector (1024 pixels). It has no
moving parts, which is the basis of the rapid scanning operating system.
The user interface is built around folders which are displayed on the home page when the instrument is switched
on. After switch on and calibration, the default home page is "Vision" offering the choice of
Applications General spectroscopic methods
Favorites A folder to store your more frequently used configured methods
Methods Contains nine folders that can store less frequently used configured methods (nine
methods per folder)
Utilities Instrument set up (date, time, language, etc) and games
Life Science Standard Life Science methods such as nucleic acid assays, protein assays and cell
counting
The instrument is supplied with a program PVC (Print via Computer) on the accompanying CD. When used with a
USB cable to connect to a PC onto which the software has been installed, it enables the user to “print through” the
PC directly to the printer that is connected to it. The data may also be stored as an Excel spreadsheet, as an EMF
graphics file, a comma delimited (csv) data file, a tab delimited (txt) data file or in native PVC format for later access
Alternatively, results may be sent to the PC via a Bluetooth accessory; this can either be supplied pre-installed or is
available as an optional accessory if the need for its use arises after installation of the product. PVC works in a
similar way.
A printer is available for the instrument; this may either be supplied pre-installed or is available as an optional
accessory if the need for its use arises after installation of the product.
Sample handling tips
• Note that the light beam is directed from RIGHT to LEFT through the cell chamber; therefore please ensure the
cell is inserted in the correct alignment.
• The cell holder supplied with the instrument accepts standard 10 mm pathlength quartz, glass or plastic cells.
• The optical beam height is 15 mm, and the minimum volume that can be used is approximately 10μl in a
Quartz ultra-micro cell.
• 12 mm test tubes may be used (e.g. for cell cultures), however they are not recommended as higher quality
data is produced by using disposable cuvettes for the analysis. If used, align the indicator line on 12 mm test
tubes in the same direction to ensure reproducible positioning of the tube. Note that test tubes do not last
forever, and that the surface becomes scratched and blemished through repetitive use; if this is the case they
should be replaced.