Hoefer SP-2001 Vision Life Spectrophotometer - Manual (Page 33)

Version 1.0
Page 33
3: Oligo
The procedure is as follows:
Step 1
Press 3 to select Oligo mode.
Step 2
Select path length using the left and right arrows. Options are 5
or 10 mm.
Press the down arrow.
Step 3 (dilution factor known)
Enter the dilution factor using the keypad numbers. Range 1.00
to 9999. Use the C button to backspace and clear the last digit
entered.
OR
Step 3 (calculate dilution factor)
Press ::; to enter the dilution factor screen.
Enter the volume of the sample using the keypad numbers.
Range 0.01 to 9999.
Press the down arrow.
Enter the volume of the diluent using the keypad numbers.
Range 0.01 to 9999.
Press
to calculate the dilution factor and return to the
Parameters screen.
OR Press
to cancel the selections and return to the
Parameters screen.
Step 4
Select whether the background correction at 320 nm is used or
not with the left and right arrows.
Press the down arrow.
Step 5
Select the units of measurement using the left and right arrows.
Options: μg/ml, ng/μl, μg/μl and pmol/μl. If pmol/μl is selected the
factor changes to a selection table denoting the ratios of the 4
bases in the structure.
Press the down arrow.
Step 6 (units not pmol/μl)
Enter the factor using the keypad numbers. Default value is 33,
range is 0.01 to 9999.
OR
Step 6 (units pmol/μl)
Enter the proportions of bases present using the keypad
numbers and up and down arrows to move between boxes.
Default is 10 for each, range is 0 to 9999.
Step 7
Press OK
to enter the Results screen
OR
Cancel
to return to the Nucleic Acids folder