Hoefer SP-2001 Vision Life Spectrophotometer - Manual (Page 38)

Version 1.0
Page 38
2: BCA
The procedure is as follows:
Step 1
Press 2 to select BCA mode.
Step 2
Wavelength for this stored method is pre-set to 562nm
Step 3
Enter the number of standard concentration points (1-9) to be
used in the curve using the keypad numbers or left and right
arrows.
Press the down arrow.
Step 4
Units: The user can enter a text string up to 8 characters
long. To access a list of pre-defined units press the Options
key ::; and then use the left/right arrows (μg/ml, μg/μl,
pmol/μl, mg/dl, mmol/l, μmol/l, g/l, mg/l, μg/l, U/l, %, ppm,
ppb, conc or none). These units can also be edited once OK
is pressed.
This screen also allows the number of displayed decimal
points (DP) to be selected, from 0 to 2 Note that the result will
always be fixed to 5 significant figures regardless of how
many decimal points are selected (so 98768.2 will display as
98768 even with 1 decimal point selected).
Press OK
to store the chosen parameters or Cancel .
Step 5
Enter the type of curve fit. Options are straight line
regression, zero regression (forces the straight line through
the origin), interpolated or cubic spline.
Press the down arrow.
Step 6
Select the calibration mode, either standards (measure
prepared standards) or manual (keypad data entry)
Step 7 (if standards selected)
Select the number of replicates using the left and right
arrows. This determines the number of standards to be
measured and averaged at each standard concentration
point. Can be OFF (1), 2 or 3.
Step 8
Press Next
to enter the Standards screen
OR
Press Cancel
to cancel selections and return to the
Protein folder.
Standards Screen
Step 9
Enter the concentration values by using the keypad numbers
and the up and down arrows to move between the different
standard boxes. Range 0.001 to 9999. C button backspaces
and clears the last digit entered.
Step 10
Press Next
to enter the Calibration screen. If there are
duplicate or non-monotonic (increasing) entries the unit will
beep and highlight the incorrect entry.
OR
Press Back
to return to the Parameter screen.