Hoefer SP-2001 Vision Life Spectrophotometer - Manual (Page 36)

Version 1.0
Page 36
1: Protein UV
This is the Christian and Warburg assay discussed previously. The procedure is as follows:
Step 1
Press 1 to select Protein UV mode.
Step 2
Select path length using the left and right arrows. Options are 5
or 10 mm.
Press the down arrow.
Step 3 (dilution factor known)
Enter the dilution factor using the keypad numbers. Range 1.00
to 9999. Use the C button to backspace and clear the last digit
entered.
OR
Step 3 (calculate dilution factor)
Press ::; to enter the dilution factor screen.
Enter the volume of the sample using the keypad numbers.
Range 0.01 to 9999.
Press the down arrow.
Enter the volume of the diluent using the keypad numbers.
Range 0.01 to 9999.
Press
to calculate the dilution factor and return to the
Parameters screen.
OR Press
to cancel the selections and return to the
Parameters screen.
Step 4
Select whether the background correction at 320 nm is used or
not with the left and right arrows.
Press the down arrow.
Step 5
Enter co-efficient 1 (280 nm) using the keypad numbers. Default
value is 1.55, range is 1.00 to 9999.
Press the down arrow.
Step 6
Enter co-efficient 2 (260 nm) using the keypad numbers. Default
value is 0.76. Range is 1.00 to 9999.
Press the down arrow.
Step 7
Select the units of measurement using the left and right arrows.
Options: μg/ml, ng/μl and μg/μl.
Step 8
Press OK
to enter the Results screen
OR
Cancel
to return to the Protein folder