Hoefer SP-2001 Vision Life Spectrophotometer - Manual (Page 3)

Version 1.0
Page 3
TABLE OF CONTENTS
ESSENTIAL SAFETY NOTES .................................................................................................................................... 4
Unpacking, Positioning and Installation .................................................................................................... 4
INTRODUCTION.......................................................................................................................................................... 5
Your spectrophotometer .............................................................................................................................. 5
Sample handling tips.................................................................................................................................... 5
Keypad and display ...................................................................................................................................... 6
Software style................................................................................................................................................ 8
THE APPLICATIONS FOLDER .................................................................................................................................. 9
1: Single Wavelength – Abs and %T ......................................................................................................... 10
2: Concentration.......................................................................................................................................... 12
3: Wavescan ................................................................................................................................................ 14
4: Simple Kinetics ....................................................................................................................................... 17
5: Standard Curve ....................................................................................................................................... 20
6: Multiple Wavelength ............................................................................................................................... 24
7: Absorbance Ratio ................................................................................................................................... 25
THE LIFE SCIENCE FOLDER .................................................................................................................................. 27
1: DNA................................................................................................................................................... 29
2: RNA................................................................................................................................................... 31
3: Oligo .................................................................................................................................................. 33
Protein Determination ................................................................................................................................ 35
1: Protein UV......................................................................................................................................... 36
2: BCA ................................................................................................................................................... 38
3: Bradford............................................................................................................................................. 41
4: Lowry................................................................................................................................................. 44
5: Biuret ................................................................................................................................................. 47
Bacterial Cell Culture Measurement (OD600) .......................................................................................... 50
FAVORITES AND METHODS FOLDERS ................................................................................................................ 52
UTILITIES FOLDER .................................................................................................................................................. 53
Utilities ......................................................................................................................................................... 54
1: Date and Time................................................................................................................................... 54
2: Regional ............................................................................................................................................ 54
3: Printer................................................................................................................................................ 54
4: Preferences....................................................................................................................................... 55
5: Contrast............................................................................................................................................. 55
6: Folder Names.................................................................................................................................... 55
7: About ................................................................................................................................................. 56
8: Games............................................................................................................................................... 56
ACCESSORIES INSTALLATION ............................................................................................................................. 58
Printer installation ...................................................................................................................................... 58
Loading / changing the printer paper ....................................................................................................... 60
Bluetooth accessory installation............................................................................................................... 61
PRINT VIA COMPUTER............................................................................................................................................ 64
ACCESSORIES ......................................................................................................................................................... 65
Lamp Replacement ..................................................................................................................................... 65
Cleaning and general care of the instrument........................................................................................... 65
SPECIFICATION AND WARRANTY ........................................................................................................................ 66