Hoefer SP-2001 Vision Life Spectrophotometer - Manual (Page 22)

Version 1.0
Page 22
Calibration Screen (replicates on)
This shows the calibration values and allows standards to be
measured.
Step 10
Insert the reference. Press 0A/100% key.
This will be used for all subsequent samples until changed.
Step 11
Press
to display the replicate entry boxes. Use C to clear
previously stored results before measuring.
Insert the standard and press Enter to measure the standard and
store the result.
Repeat for all replicates and standards.
A graph will display the results and the fitted curve as the
measurements are input.
Use the up and down arrows to select a standard to be repeated
if a poor reading has been obtained. Use C to clear the previous
reading.
Step 12
Press
to accept the calibration and go to the Results screen
(see below)
OR
Press Back
to return to the Standards screen.
Calibration (Manual entry)
Shows previously entered calibration values and allows values to
be entered via the keypad.
The highlighted box can be edited in order to enter an
absorbance value corresponding to a given concentration value
using the keypad numbers. Range 0.001 to 9999. Use C to
backspace and clear the last digit entered and the up and down
arrows to move between boxes.
Press OK
to accept the calibration and go to the Results
screen (see below)
OR
Press Back
to return to the Standards screen.
Results screen
Step 13
Insert the reference and press the 0A/100%T key. This will be
used for all subsequent samples until changed.
Step 14
Insert the sample and press
.
The concentration of the sample is taken and displayed.
Repeat step 14 for all samples.
Press
to return to the Applications Folder.
Press ::; to display available Options which are described
below.