Hoefer SP-2001 Vision Life Spectrophotometer - Manual (Page 34)

Version 1.0
Page 34
Results Screen
Step 8
Insert the reference. Press 0A/100%T Key. This will be used for
all subsequent samples until changed.
Step 9
Insert sample and press
. This measures at the selected
wavelengths and displays the results. The ratio of wavelengths 1
and 2 absorbencies are calculated (both corrected by the
background wavelength value if selected). Gives concentration
based on absorbance at wavelength 1.
Repeat step 9 for all samples.
Press
to return to the Nucleic acid folder.
Press ::; to display available Options which are described
below.
Options (select using key pad numbers)
1. Return to parameters screen (step 1 above).
2. Print result via selected method.
3. Toggle graph on/off. The graph shows a wavescan plot
across the range 220 nm to 320 nm with cursors denoting
230, 260, 280 and (if background correction selected) 320
nm.
7. Sample number – add a prefix to the sample number and
reset the incrementing number to the desired value.
8. Save method – use the left and right arrows to select a folder
to store in (Favorites/Methods 1-9), press the down arrow
and enter name.
9. Auto-print – toggles auto-print on/off.
Exit options by pressing
, or wait.