UVP 81-0345-01 ChemiDoc-ItTS2 Imager - Manual (Page 24)

GelDoc-It
TS2
and ChemiDoc-It
TS2
Imagers 24
“Compositing” Tab
To access image compositing tools, press to select the
Compositing tab.
Copositing tools include Fluorescent, Merge and
Chemiluminescent. Press to select the desired compositing
tool once the Compositing tab has been selected.
Fluorescent Compositing Tool
The Fluorescent Compositing Tool is used to merge
various fluorescently-colored channels into one image.
To use this tool, first open two or three images in the gray
area (gallery bar) below the “Compositing” tab. Drag the
base/primary image from the gallery bar to the “Drag
image here” Base Image area. Then, drag one or two
overlay images to the second and third “Drag image here”
boxes.
The base and overlay images’ histogram, pseudocolor and
inversion attributes can be adjusted to create the ideal
composited image. To do so, select the desired image to
be adjusted by pressing it. A black outline will surround the
selected image. Then, use the Histogram, Pseudocolor
and Invert tools on the left side of the screen to modify the
image(s) and Preview composited image as desired.
Use the percentage tool to the right of the overlay image(s)
to determine the intensity of the overlay image(s) in the
final composited image. Note that the percentage shown
pertains to the saturation levels in the overlay image(s).
Therefore, as higher percentages are selected, only pixels
with higher saturation levels will be shown in the final
composited image. For example, if 65% is selected, only
pixels with over 65% saturation in the overlay image will be
shown in the final composited image.
Once the ideal composited image has been created, press
the Checkmark button below the Preview composited
image to complete fluorescent compositing.
Merge Compositing Tool
The Merge Compositing Tool is used to merge up to
three different images, pulling a specific color channel (red,
green or blue) from each image. This tool is useful for
multiplexing applications.
The Merge Compositing Tool consists of three gray
rectangles where images can be placed (each rectangle
states “Drag image here”). The color channel to be pulled
from the image in each box is shown in the colored bar
below the gray box (red on top, green in the middle, and
blue on the bottom).
To use this tool, first open two or three images in the gray
area (gallery bar) below the “Compositing” tab. Depending
upon the desired color to be composited, drag an image
from the gallery bar to the “Drag image here” area.
Each image’s histogram, pseudocolor and inversion
attributes can be adjusted to create the ideal composited
image. To do so, select the desired image to be adjusted
by pressing it. A black outline will surround the selected
image. Then, use the Histogram, Pseudocolor and Invert
tools on the left side of the screen to modify the image(s)
and the Preview composited image as desired.