Hanna Instruments HI 83099 User Manual
Page 26
51
50
Copper LR
• Remove the cuvette.
• Add the content of one packet of HI 95747-0 Bicinchoninate.
Replace the cap and shake gently for about 15 seconds.
• Reinsert the cuvette into the instrument.
• Press
Timer and the display will show the countdown prior to the measurement or, alternatively, wait
for 45 seconds and press
Read. When the timer ends the meter will perform the reading.
• The instrument displays the results in
µµµµµ
g/L of copper.
INTERFERENCES
Interference may be caused by:
Silver
Cyanide
For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted between
6 and 8.
Copper LR
COPPER LOW RANGE
SPECIFICATIONS
Range
0 to 1000
µ
g/L
Resolution
1
µ
g/L
Accuracy
±10
µ
g/L ±5% of reading at 25 °C
Typical EMC
±1
µ
g/L
Deviation
Light Source
Tungsten lamp with narrow band interference filter @ 575 nm
Method
Adaptation of the
EPA method. The reaction between copper and the bicinchoninate
reagent causes a purple tint in the sample.
REQUIRED REAGENTS
Code
Description
Quantity
HI 95747-0
Bicinchoninate
1 packet
REAGENT SETS
HI 95747-01 Reagents for 100 tests
HI 95747-03 Reagents for 300 tests
For other accessories see page 132.
MEASUREMENT PROCEDURE
• Select the
Copper LR method using the procedure described in
the
Method Selection section (see page 12).
• Fill the cuvette with 10 mL of unreacted sample (up to the
mark) and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the
Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement.
10 mL