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Hanna Instruments HI 83099 User Manual

Page 26

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51

50

Copper LR

• Remove the cuvette.

• Add the content of one packet of HI 95747-0 Bicinchoninate.

Replace the cap and shake gently for about 15 seconds.

• Reinsert the cuvette into the instrument.

• Press

Timer and the display will show the countdown prior to the measurement or, alternatively, wait

for 45 seconds and press

Read. When the timer ends the meter will perform the reading.

• The instrument displays the results in

µµµµµ

g/L of copper.

INTERFERENCES

Interference may be caused by:
Silver
Cyanide
For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted between
6 and 8.

Copper LR

COPPER LOW RANGE

SPECIFICATIONS

Range

0 to 1000

µ

g/L

Resolution

1

µ

g/L

Accuracy

±10

µ

g/L ±5% of reading at 25 °C

Typical EMC

±1

µ

g/L

Deviation
Light Source

Tungsten lamp with narrow band interference filter @ 575 nm

Method

Adaptation of the

EPA method. The reaction between copper and the bicinchoninate

reagent causes a purple tint in the sample.

REQUIRED REAGENTS

Code

Description

Quantity

HI 95747-0

Bicinchoninate

1 packet

REAGENT SETS

HI 95747-01 Reagents for 100 tests
HI 95747-03 Reagents for 300 tests
For other accessories see page 132.

MEASUREMENT PROCEDURE

• Select the

Copper LR method using the procedure described in

the

Method Selection section (see page 12).

• Fill the cuvette with 10 mL of unreacted sample (up to the

mark) and replace the cap.

• Place the cuvette into the holder and close the lid.

• Press the

Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement.

10 mL