Bio-Rad Image Lab™ Software User Manual
Page 81

Regression Methods
User Guide | 81
The following tables list the light type and filter to be used with various stains for
each application for the GS-900 densitometer.
Table 4. Imaging gels on the GS-900 calibrated densitometer
Stain
Light Type
Filter
Coomassie G-250 (colloidal)
Transmissive
Red
Coomassie G-250 (Bio-Safe™)
Transmissive
Red
Coomassie R-250
Transmissive
Red
Silver
Transmissive
Green
Copper
Transmissive
Red
Zinc
Transmissive
Green
Table 5. Imaging blots on the GS-900 calibrated densitometer
Detection Reagent
Light Type
Filter
Coomassie
Reflective
Red
Colloidal gold
Reflective
Green
Amido black
Reflective
Green
BCIP/NBT AP-substrate
Reflective
Green
4CN HRP-substrate
Reflective
Red
DAB HRP-substrate
Reflective
Green
Color blot
Reflective
Green
Table 6. Imaging X-ray film on the GS-900 calibrated densitometer
Film
Light Type
Filter
Blue film
Transmissive
Blue
Gray film
Transmissive
Red