6 analyzing images, Using auto analysis, Chapter 6 analyzing images – Bio-Rad Image Lab™ Software User Manual
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User Guide | 103
6
Analyzing Images
Analysis Tool Box tools are enabled when an image file is opened and in focus. An
active or “in focus” window has a darker blue menu bar on a Windows PC. On a
Macintosh, the window control icons display more brightly when a window is active.
This distinction helps you to identify the active window among many open image
files in your workspace.
Using Auto Analysis
Auto analysis detects the lanes and bands and, optionally, calculates the molecular
weight of the bands in your image. Click Auto-Analysis in the Analysis Tool Box to
do the following:
Analyze images obtained with protocols that did not include steps for auto
detection and analysis
Change your analysis parameters to reanalyze your images
Note: If you change any settings for an analyzed gel, the initial analysis is
overwritten. To preserve both analyses, save each image file with a different
name.