Bio-Rad Image Lab™ Software - Manual (Page 127)

Quantity Tools
User Guide | 127
Linear — generates a straight line that is the best fit of the values you provided
and is preferred in most cases.
Point-to-point — generates a curve in which each data point is connected
directly to the next, regardless of the shape of the resulting curve.
Cubic spline — generates a smooth curve that connects each data point. At
least four standard points are required to use this method of least-squares
polynomial fits.
6. Click Standard Curve on the toolbar and select the Absolute Quantity Standard
Curve tab.
Table 7. Regression Methods
Regression
Method Minimum Number
of Standard Bands Minimum Number with Force
Through Origin Option
Linear 2 1
Point-to-point 2 1
Cubic spline 5 4