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Step 4. specify output – Bio-Rad Image Lab™ Software User Manual

Page 72

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4 | Acquiring Images

72 | GS-900 Calibrated Densitometer with Image Lab Software

Gradient gels — the linear (semilog) regression method works well for

these gels because the mobility of the bands is linear to the log of their

molecular weight. As an alternative, the point-to-point (semilog) method

can be used if the R

2

value is not sufficient.

Fixed percentage gels — these gels have a nonlinear relationship

between the gels’ mobility and their molecular weight. For these gels,

choose the logistic or cubic spline regression method.

For more information about regression methods, see

Regression Methods on

page 80

.

Step 4. Specify Output

To specify the output of the protocol

1.

Select Specify Output in the left pane of the Protocol Setup window to display
output options.

2.

In the right pane, you can choose to

Automatically print the image

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