Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 50)

44 Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
TBE-urea (30 ml) 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8 .0, 12%
(catalog #161-0768) Ficoll, 0 .01% bromophenol blue, 0 .02% xylene cyanole,
Store at 4°C 7 M urea
Tris base 0.32 g
Boric acid 0.165 g
EDTA 17.5 mg
Ficoll 3.6 g
Bromophenol blue 3 mg
Xylene cyanole FF 6 mg
Urea 12.6 g
diH
2
O to 30 ml
Buffer Components
0 .5 M Tris-HCl, pH 6 .8 (1 L) Tris base 60.6 g
(catalog #161-0799) diH
2
O ~900 ml
Store at 4°C Adjust to pH 6.8 with HCl
diH
2
O to 1 L
10% SDS (250 ml) SDS 25.0 g
(catalog #161-0416) diH
2
O to 250 ml
1 .0% Bromophenol blue (10 ml) Bromophenol blue 100 mg
(10 g powder, catalog #161-0404) diH
2
O to 10 ml
Blotting Buffers
Towbin buffer (1 L) 25 mM Tris, 192 mM glycine, 20% methanol
Dissolve: Tris base 14.4 g
Glycine 3.03 g
diH
2
O 500 ml
Then add: Methanol 200 ml
diH
2
O to 1 L
Alternatively, use: 10x Tris/glycine (catalog #161-0734) 100 ml
Add 200 ml methanol and diH
2
O to 1 L as above
Tris-buffered saline with 20 mM Tris, 500 mM NaCl, 0 .05% Tween 20
Tween (TTBS, 1 L)
Tris base 2.4 g
NaCl 29.2 g
10% Tween 20 5.0 ml
diH
2
O to 1 L
Alternatively, use: 10x TBS (catalog #170-6435) 100 ml
10% Tween 20 (catalog #166-2404) 5 ml
diH
2
O 895 ml
Criterion Precast Gels