4 sample preparation, 5 running conditions – Bio-Rad Criterion™ TBE-Urea Precast Gels User Manual
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8.4 Sample Preparation
1. Determine the appropriate protein concentration of your sample based on the detection method
and load volume used. (See Chapter 12 for approximate stain sensitivities.)
2. Dilute 1 part sample with 1 part sample buffer. Do not heat the samples.
8.5 Running Conditions
Power conditions
125 V constant
Starting current
90–120 mA/gel
Final current
35–55 mA/gel
Run time
90 min
Criterion Precast Gels
This manual is related to the following products:
- Criterion™ TBE Precast Gels Criterion™ Cell Criterion Precast Gels Criterion Dodeca Cell 2-D Electrophoresis Workflow Criterion™ IEF Precast Gels Criterion™ Zymogram Precast Gels Criterion™ XT Tris-Acetate Precast Gels Criterion™ Tris-Tricine Precast Gels Criterion™ XT Bis-Tris Precast Gels Criterion Stain Free™ Tris-HCl Gels Criterion™ Tris-HCl Precast Gels Criterion™ TGX Stain-Free™ Precast Gels Criterion™ TGX™ Precast Gels