1 introduction, 2 criterion tbe gels, 1 gel composition – Bio-Rad Criterion™ TBE-Urea Precast Gels User Manual
Page 31: 2 gel selection guide, Nondenaturing nucleic acid page, 3 nondenaturing nucleic acid page buffers

Nondenaturing Nucleic
Acid PAGE
9
9.1 Introduction
Criterion
™
TBE gels are used to separate small double-stranded DNA (dsDNA) fragments, particularly
PCR products. DNA molecules have nearly uniform mass-to-charge ratios, allowing nondenaturing
nucleic acid PAGE to separate dsDNA by mass using a continuous TBE buffer system.
9.2 Criterion TBE Gels
9.2.1 Gel Composition
Gel buffer
89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.3
Crosslinker
3.3% C
Stacking gel
4% T, 3.3% C
Storage buffer
89 mM Tris, 89 mM boric acid, 2 mM EDTA
Shelf life
~12 weeks at 2–8°C; expiration date is printed on each cassette
9.2.2 Gel Selection Guide
Gel Percentage
Optimum Separation Range
5%
200–2,000 bp
10%
50–1,500 bp
15%
20–1,000 bp
4–20%
10–2,000 bp
9.3 Nondenaturing Nucleic Acid PAGE Buffers
See Appendix B for buffer formulations. Do not adjust pH unless directed to do so.
Running buffer (1x)
89 mM Tris, 89 mM boric acid, 2 mM EDTA
Dilute 100 ml 10x stock (catalog #161-0733) with 900 ml diH
2
O
Sample buffer (5x)
50 mM Tris-HCl, pH 8.0, 5 mM EDTA, 25% glycerol, 0.2% bromophenol
(catalog #161-0767)
blue, 0.2% xylene cyanole FF
Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
25
- Criterion™ TBE Precast Gels Criterion™ Cell Criterion Precast Gels Criterion Dodeca Cell 2-D Electrophoresis Workflow Criterion™ IEF Precast Gels Criterion™ Zymogram Precast Gels Criterion™ XT Tris-Acetate Precast Gels Criterion™ Tris-Tricine Precast Gels Criterion™ XT Bis-Tris Precast Gels Criterion Stain Free™ Tris-HCl Gels Criterion™ Tris-HCl Precast Gels Criterion™ TGX Stain-Free™ Precast Gels Criterion™ TGX™ Precast Gels