3 criterion xt bis-tris gels, 3 criterion, Xt bis-tris gels – Bio-Rad Criterion™ TBE-Urea Precast Gels User Manual
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3.2.3 Criterion XT Bis-Tris Gels
Criterion XT Bis-Tris gels are based on a Bis-Tris HCl buffer system (pH 6.4) that uses discontinuous
chloride and MES or MOPS ion fronts to form moving boundaries that stack and separate denatured
proteins by size. This chemistry of XT Bis-Tris gels allows maximum stability and consistent results with
a shelf life of at least 12 months.
Running XT Bis-Tris gels with either XT MES or XT MOPS denaturing running buffer produces different
migration patterns. A combination of these two running buffers and three XT Bis-Tris gels can generate
up to six different migration patterns for small to midsize proteins.
Gel Composition
Gel buffer
Bis-Tris HCl, pH 6.4
Crosslinker
5% C
Stacking gel
4% T, 5% C
Storage buffer
Bis-Tris HCl, pH 6.4
Shelf life
12 months at ambient temperature; expiration date is printed on each cassette
Migration charts for protein standards on Criterion Tris-HCl, Criterion TGX, and TGX Stain-Free gels.
Criterion Precast Gels
- Criterion™ TBE Precast Gels Criterion™ Cell Criterion Precast Gels Criterion Dodeca Cell 2-D Electrophoresis Workflow Criterion™ IEF Precast Gels Criterion™ Zymogram Precast Gels Criterion™ XT Tris-Acetate Precast Gels Criterion™ Tris-Tricine Precast Gels Criterion™ XT Bis-Tris Precast Gels Criterion Stain Free™ Tris-HCl Gels Criterion™ Tris-HCl Precast Gels Criterion™ TGX Stain-Free™ Precast Gels Criterion™ TGX™ Precast Gels