Bio-Rad Criterion™ TBE-Urea Precast Gels User Manual
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Table 13 .1 . Placement of cassettes in the Trans-Blot Turbo cell .
Acceptable
Unacceptable
Option 1
Option 2
Option 1
Option 2
Upper bay (A)
1 mini gel
2 mini gels -or- 1 midi gel
1 mini gel
2 mini gels -or- 1 midi gel
-and/or-
-and/or-
-and-
-and-
Lower bay (B)
1 mini gel
2 mini gels -or- 1 midi gel
2 mini gels -or- 1 midi gel
1 mini gel
Table 13 .2 . Trans-Blot Turbo transfer protocols .
Protocol Name
MW, kD
Time, Min
1 Mini Gel
2 Mini Gels or 1 Midi Gel
STANDARD SD
Any
30
Up to 1.0 A, 25 V constant
Up to 1.0 A, 25 V constant
1.5 MM GEL
Any
10
2.5 A constant, up to 25 V
1.3 A constant, up to 25 V
HIGH MW
>150
10
2.5 A constant, up to 25 V
1.3 A constant, up to 25 V
LOW MW
<30
5
2.5 A constant, up to 25 V
1.3 A constant, up to 25 V
MIXED MW
5–150
7
2.5 A constant, up to 25 V
1.3 A constant, up to 25 V
1 Mini TGX
5–150
3
2.5 A constant, up to 25 V
N/A
Refer to the Trans-Blot Turbo Instruction Manual (bulletin 10020688) for complete instructions or the
Protein Blotting Guide (bulletin 2895) for additional information.
Top ion reservoir stack
Gel
Cassette top
(–) electrode (cathode)
Membrane
Cassette bottom
(+) electrode (anode)
Bottom ion reservoir
stack
Assembly of the gel blot sandwich with the Trans-Blot Turbo system .
Instruction Manual and Application Guide
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