Bio-Rad Criterion™ TBE-Urea Precast Gels - Manual (Page 48)

Buffers
B
Running Buffers
10x SDS-PAGE (1 L) 250 mM Tris, 1 .92 M glycine, 1% SDS, pH 8 .3
(catalog #161-0732)
Tris base 30.3 g
Glycine 144.1 g
SDS 10 g
diH
2
O to 1 L
Do not adjust the pH (~pH 8.3)
10x Native PAGE (1 L) 250 mM Tris, 1 .92 M glycine, pH 8 .3
(catalog #161-0734)
Tris base 30.3 g
Glycine 144.1 g
diH
2
O to 1 L
Do not adjust the pH (~pH 8.3)
10x Tris-Tricine (1 L) 1 M Tris, 1 M Tricine, 1% SDS, pH 8 .3
(catalog #161-0744)
Tris base 121.1 g
Tricine 179.2 g
SDS 10 g
diH
2
O to 1 L
Do not adjust the pH (~pH 8.3)
10x TBE (1 L) 890 mM Tris, 890 mM boric acid, 20 mM EDTA
(catalog #161-0741)
Tris base 107.8 g
Boric acid 55.0 g
EDTA 5.8 g
diH
2
O to 1 L
Do not adjust the pH (~pH 8.3)
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