Chapter 12: detection, Analyze the separation, Chapter 12) – Bio-Rad Criterion™ TBE-Urea Precast Gels User Manual
Page 36: Stain the gels for total protein, Detection, 1 sds-page and native page detection

Detection
12
12.1 SDS-PAGE and Native PAGE Detection
Following electrophoresis, either stain the gel or use the Criterion Stain Free
™
system to visualize
proteins in the gel.
n
Refer to Table 12.1 for a comparison of total protein stains
n
For Criterion
™
TGX Stain-Free
™
and Criterion Stain Free
™
gels, immediately place the gel on
the tray of the Gel Doc
™
EZ imager; no additional fixation or rinsing steps are required.
If desired, stain with any compatible stains (Table 12.1) following imaging. Some stains, if
used prior to imaging, can impair imaging quality
Table 12 .1 . Total protein gel stains for use with Criterion gels .
Stain
Sensitivity
(Lower
Limit)
Optimum
Protein Load
(µg/Band)
Advantages
Disadvantages
Imaging
Manual
Criterion Gels
Coomassie
R-250
36–47 ng
~0.5
Laboratory
standard
Requires
methanol
destaining
Photography
with white light
or transmission
densitometry
Consult
literature
Bio-Safe
™
Coomassie
8–28 ng
~0.5
Nonhazardous
4307051
Zinc stain
1
6–12 ng
~0.2
High contrast, fast,
reversible
Negative SDS-
PAGE stain, must
be photographed
4006082
Silver
Stain Plus
™
kit
0.6–1.2 ng
~0.01
Sensitive, robust,
mass spectrometry
compatible
Does not stain
glycoproteins
well
LIT442
Silver stain
0.6–1.2 ng
~0.01
Stains complex
proteins (glyco- or
lipoproteins)
Not mass
spectrometry
compatible
LIT34
Dodeca
™
silver stain kit
0.5–1.2 ng
~0.1
Convenient
staining for a large
number of gels
4110150
Oriole
™
fluorescent
gel stain
1
~2 ng
~0.1
High sensitivity,
broad dynamic
range, simple
one-step protocol
Fluorescence
visualization
with UV transil-
lumination
10017295
1
Do not use zinc stain or Oriole fluorescent gel stain to stain native gels.
30
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