Bio-Rad Gene Pulser Xcell™ Electroporation Systems - Manual (Page 5)

6.3.3 Solutions and Reagents ................................................................57
6.4 Bacillus cereus ............................................................................................57
6.4.1 Preparation of Electrocompetent Cells............................................57
6.4.2 Electroporation ..............................................................................57
6.4.3 Solutions and Reagents ................................................................58
6.5 Pseudomonas aeruginosa ..........................................................................58
6.5.1 Preparation of Electrocompetent Cells............................................58
6.5.2 Electroporation ..............................................................................59
6.5.3 Solutions and Reagents ................................................................59
6.6 Streptococcus pyogenes ............................................................................60
6.6.1 Preparation of Electrocompetent Cells............................................60
6.6.2 Electroporation ..............................................................................60
6.6.3 Solutions and Reagents ................................................................61
6.7 Lactobacillus plantarum ..............................................................................61
6.7.1 Preparation of Electrocompetent Cells............................................61
6.7.2 Electroporation ..............................................................................61
6.7.3 Solutions and Reagents ................................................................62
Section 7 Electroporation of Fungal Cells..............................................................62
7.1 Saccaromyces cerevisiae ............................................................................62
7.1.1 Preparation of Electrocompetent Cells............................................62
7.1.2 Electroporation ..............................................................................63
7.1.3 Solutions and Reagents ................................................................64
7.2 Schizosaccharomyces pombe ....................................................................64
7.2.1 Preparation of Electrocompetent Cells............................................64
7.2.2 Electroporation ..............................................................................65
7.2.3 Solutions and Reagents ................................................................65
7.3 Pichia pastoris ............................................................................................65
7.3.1 Preparation of Electrocompetent Cells............................................65
7.3.2 Electroporation ..............................................................................66
7.3.3 Solutions and Reagents ................................................................66
7.4 Candida albicans ........................................................................................67
7.4.1 Preparation of Electrocompetent Cells............................................67
7.4.2 Electroporation ..............................................................................67
7.4.3 Solutions and Reagents ................................................................68
7.5 Dictyostelium discoideum............................................................................68
7.5.1 Preparation of Electrocompetent Cells............................................68
7.5.2 Electroporation ..............................................................................69
7.5.3 Solutions and Reagents ................................................................69
Section 8 Mammalian Cells ......................................................................................70
8.1 Preparation of Electrocompetent Cells ........................................................70