Bio-Rad Gene Pulser Xcell™ Electroporation Systems - Manual (Page 3)

Table of Contents
Section 1 Introduction and Safety Information: The Gene Pulser Xcell
System ........................................................................................................1
1.1 General Safety Information ............................................................................1
1.2 Electrical Hazards ........................................................................................2
1.3 Mechanical Hazards......................................................................................2
1.4 Other Safety Precautions ..............................................................................2
Section 2 Unpacking and System Installation ........................................................3
2.1 Unpacking the System Components ............................................................3
2.2 Setting up the System ..................................................................................4
2.2.1 Setting up the Gene Pulser Xcell Main Unit and Connecting
the ShockPod (Cat. #s 165-2660, 165-2661, 165-2662,
165-2666) ........................................................................................4
2.2.2 Connecting the PC Module to the Gene Pulser Xcell Main
Unit (Cat. #s 165-2660, 165-2662, and 165-2668) ..........................5
2.2.3 Connecting the CE Module to the Gene Pulser Xcell Main
Unit (Cat. #s 165-2660, 165-2661, and 165-2667) ..........................6
2.2.4 ShockPod (Cat. #s 165-2660, 165-2661, 165-2662, and
165-2669) ........................................................................................6
Section 3 Gene Pulser Xcell Operating Instructions ..............................................8
3.1 Section Overview ..........................................................................................8
3.2 Front Panel and Home Screen ......................................................................9
3.2.1 Description of Keypad......................................................................9
3.2.2 Home Screen ................................................................................10
3.2.3 Help Screens ................................................................................11
3.3 Manual Operation........................................................................................12
3.3.1 Manual Operation (Guide Guide) ....................................................12
3.3.2 Electroporation using Exponential Decay Pulses ............................12
3.3.3 Electroporation Specifying Time Constant......................................14
3.3.4 Electroporation using Square Wave Pulses ....................................15
3.3.5 Results Screens ............................................................................17
3.3.6 Saving a Program from Manual Operation......................................19
3.3.6A Saving in a Location without a Named User Entry................20
3.3.6B Saving in a Location with a Named User Entry ....................20
3.4 Pre-Set Protocols........................................................................................21
3.4.1 Using a Pre-set Protocol (Quick Guide) ..........................................21
3.4.2 Electroporation using a Pre-Set Protocol........................................22
3.4.3 Modifying Pre-Set Protocol Parameters ........................................25
3.4.4 Saving Changes to Pre-Set Protocols ............................................25
3.5 User Protocols ............................................................................................26