Bio-Rad Gene Pulser Xcell™ Electroporation Systems - Manual (Page 4)

3.5.1 Using a User Protocol (Quick Guide) ..............................................26
3.5.2 Creating a New User Name ..........................................................26
3.5.3 Creating a New User Protocol........................................................26
3.5.4 Modifying a User Protocol ..............................................................30
3.5.5 Deleting a User Name and a User Protocol....................................31
3.5.6 Renaming a User Name or a User Protocol ..................................33
3.6 Last Pulse ..................................................................................................34
3.7 Optimize Operation ....................................................................................34
3.8 Data Management ......................................................................................36
3.9 Measurements ............................................................................................39
3.9.1 Sample Resistance Measurements ................................................39
3.9.2 Calibration and Measurement of Capacitors in the
CE Module ..................................................................................................40
3.10 User Preferences ........................................................................................41
3.10.1 Setting the Clock ..........................................................................41
3.10.2 Adjusting the Screen Intensity ........................................................42
3.10.3 Sleep Function Setting ..................................................................42
3.11 The Pulse Trac System ..............................................................................43
3.11.1 Pulse Trac System Description ......................................................43
3.11.2 Pulse Trac Diagnostic Algorithm ....................................................44
Section 4 Overview of Electroporation Theory ......................................................44
4.1 Exponential Decay Pulses ..........................................................................45
4.2 Square Wave Pulses ..................................................................................45
Section 5 Factors Affecting Electroporation: Optimizing
Electroporation ........................................................................................48
5.1 Cell Growth ................................................................................................48
5.2 DNA ............................................................................................................49
5.3 Electroporation Media ................................................................................49
5.4 Temperature................................................................................................50
Section 6 Electroporation of Bacterial Cells ..........................................................52
6.1 Escherichia coli ..........................................................................................52
6.1.1 Preparation of Electrocompetent Cells............................................52
6.1.2 Electroporation ..............................................................................53
6.1.3 Solutions and Reagents ................................................................53
6.2 Staphylococcus aureus ..............................................................................54
6.2.1 Preparation of Electrocompetent Cells............................................54
6.2.2 Electroporation ..............................................................................54
6.2.3 Solutions and Reagents ................................................................55
6.3 Agrobacterium tumefaciens ........................................................................56
6.3.1 Preparation of Electrocompetent Cells............................................56
6.3.2 Electroporation ..............................................................................56