Bio-Rad S3™ Cell Sorter - Manual (Page 9)

Instruction Manual | vii
Table of Contents
Chapter 1: Introduction 1
1.1 System Components 2
1.2 Installation Requirements 2
Chapter 2: Hardware Description 3
2.1 System Overview 3
2.2 Fluidics System 5
2.3 Optics 9
2.4 Electronics 11
Chapter 3: ProSort ™ Software 13
3.1 Main Software Window 14
3.2 Control Panel 20
3.3 Administrator Tab Toolbar 22
Chapter 4: Getting Started 33
4.1 Checking Bulk Fluidics 33
4.2 Logging In 34
4.3 Daily Startup 35
4.4 Quality Control 36
4.5 Protocols and Workspace 42
Chapter 5: Acquisition 51
5.1 Acquisition Setup 51
5.2 Compensation 55
5.3 Checking or Swapping Fluidics 60
5.4 Optical Filters 62
Chapter 6: Sorting 65
6.1 Sort Setup 65
6.2 Sort Modes 69
6.3 Sort Statistics 69
6.4 Sort Plots 71
Chapter 7: Additional Software Features 73
7.1 Debubble 73
7.2 Unclog 73
7.3 Swap Tip 73
7.4 Clean System 74
7.5 Instrument Status Box 75
7.6 Status Bar 75
7.7 Printing 76
7.8 Quality Control Reports 76
7.9 User Reports 77
7.10 Biosafety System 78
Chapter 8: Shutdown 79
8.1 Daily Shutdown 79
Chapter 9: Automatic Startup 83
9.1 Scheduling an Automatic Startup 83
9.2 Previously Scheduled Automatic Startups 84
Chapter 10: Maintenance 87
10.1 General Maintenance 87
10.2 Dealing with Clogs 88
10.3 Cleaning or Replacing Nozzle Tip 88
10.4 Swap Nozzle Tip Wizard 89
10.5 Optical Filter Cleaning 105
10.6 Disinfectants 105
10.7 Decontamination 105
Troubleshooting 111
References 115
S3 and S3e Cell Sorter Specifications 117
Ordering Information 119