Bio-Rad S3™ Cell Sorter - Manual (Page 121)

Instruction Manual | 111
Troubleshooting
11
Table 30 describes possible errors and causes with troubleshooting steps.
Table 30. Troubleshooting table.
Error
Possible Causes Troubleshooting Steps
Bubble detected Bubble in sample line Place the loading dock in the wash position
to backflush the sample line and remove
the bubble
Nozzle door interlock Nozzle door is open Close nozzle door
Sort door interlock Sort door is open Close sort door
Waiting for QC QC has not been run since the Run QC to proceed with sorting
system has been started up
Not maintaining droplets Large shift in drops Perform Drop Delay to put the system back
into maintaining droplets
Cannot unlock loading stage System is still washing Wait for wash to complete
Sample is currently running Stop sort or acquisition
QC will not pass Calibration beads diluted Use the undiluted ProLine ™ beads directly
from bottle
Using wrong calibration beads Use only ProLine beads
QC criteria changed Refer to the correct bead lot file
Fluidic error message Quick disconnects are not Recheck connections. An audible click must
connected properly be heard for proper connection
Stream coming out at an angle Clog in nozzle tip Use Swap Tip Wizard and clean nozzle tip
Reseat nozzle tip on to the nozzle
Manual compensation Compensation checkbox Check and uncheck compensation
not working not checked box on the axis
Cannot see events labeled Filter configuration may Check filter configuration against
with fluorophores be incorrect fluorophore combination
Laser turned off Check that both lasers are powered
Cannot see rare events Total events displayed is Increase cycle mode value to display
too low more events
continues