Bio-Rad S3™ Cell Sorter - Manual (Page 83)

Instruction Manual | 73
Additional Software Features
7
The ProSort ™ software is an acquisition and sorting software package with the additional
features described in this chapter.
7.1 Debubble
During the startup procedure, a debubble step will occur to clear the system of air. At times,
a bubble may enter the nozzle and cause problems with the stream. On the Setup and
Maintenance tab select Debubble to rinse the system and attempt to debubble the nozzle. The
stream can also be monitored at this time to see any changes.
7.2 Unclog
A clog can easily be introduced and can cause multiple problems with the fluidics. When a
clog occurs, the software will display an error, stop the sample, stop the sort, and eject sorted
samples for protection.
Use the Unclog button on the Setup and Maintenance tab toolbar as a first-line action. The
S3
™ and S3e ™ Systems have the ability to backflush the nozzle, potentially releasing the clog
and flushing it into the waste. If successful, run a couple of debubble cycles to remove any air
that may have been pulled into the nozzle body.
IMPORTANT! To avoid clogs when working with cells, resuspend cells into single
cell suspension and always use a 40 µm or smaller filter prior to cell sorting.
7.3 Swap Tip
There will be occasions when the unclog function will not be able to clear a clog in the system.
Use the Swap Tip button on the Setup and Maintenance tab toolbar to run the Swap Tip
wizard. This wizard will walk through each step of the nozzle tip swap. Use the same Swap Tip
button when nozzle adjustments are necessary or the nozzle needs cleaning.
Please refer to Section 10.4, Swap Nozzle Tip Wizard, for step-by-step instructions on
swapping the nozzle tip, or contact Technical Support if any of these solutions do not resolve
the problems.