Bio-Rad S3™ Cell Sorter - Manual (Page 112)

102 | S3 and S3e Cell Sorters
Maintenance
Fig. 122. Adjust Stream Position window.
29. Using the Pitch and Roll arrows, adjust the stream’s position until the stream is hitting the
waste hole.
30. Click the blue forward arrow.
31. Close the sort chamber door.
32. Using the Pitch and Roll arrows, move the stream until it is visible as a vertical white line in
the camera image on the right of the window (Figure 12).
33. Adjust the stream so that the red dotted lines become green.
34. Click the blue forward arrow.
Note: When the stream is in line with the stream laser, the illuminated stream will become
thicker and more visible.
35. Using the Vertical, Horizontal, and Focus arrows, adjust the nozzle tip and stream until it
matches the last aligned image (Figure 123).
36. If there appears to be a clog or if the stream appears to be uneven, repeat cleaning the tip
and reseating the nozzle as described starting on step 1.
37. If the nozzle tip and stream are aligned and there are no clogs, click the checkmark to
proceed (Figure 123).