Bio-Rad S3™ Cell Sorter - Manual (Page 40)

30 | S3 and S3e Cell Sorters
ProSort Software
Boost Adjustment
The default boost duration is optimized to bring the sample into position for interrogation by
the laser once acquisition is started. Default values can be restored by selecting Default. If this
value is adjusted, test the boost duration with a known sample to optimize the duration before
running the unknown samples.
Force Cleaning
There are two methods to end a user session in the software — log out or shut down. For both
methods, a Force Cleaning option is available to promote cleaning and maintenance of the
system. Force cleaning can help to avoid unnecessary clogs that can happen when the system
is not cleaned regularly or left overnight with sample in the lines.
Default Plot Statistics
Each plot can be displayed with statistics for all events or regions drawn on the plot. Each
statistic can be displayed or can be hidden by setting the preference. In addition, the number
of significant figures for each statistic can be defined. Once this parameter is changed the
subsequent new plots will reflect the new statistics defaults.
Plot Defaults
In this section there are several options that can be chosen.
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Histogram and Density Plot axis parameters can be defined and set in the global
preferences. The user can choose the default axis parameter type: Area, Height, or Width. In
addition, the axis can be displayed in log or linear scales. Log can be selected as the default
by checking the box. Once these parameters are changed, subsequent new plots will reflect
the new parameter defaults
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The width and height of the plot can be defined specifically. The default number of pixels
is 400 x 400. In addition, the aspect ratio can be maintained if the box is checked for this
option. This option applies to both histograms and density plots. Once this parameter is
changed, subsequent new plots will reflect the new size default
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Events may fall off scale and be displayed on or below the axis. A preference can be chosen
to show an arrow indicator when events are off scale. This will help to raise awareness that
some settings may need to be adjusted
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Auto compensation is the recommended method to compensate samples properly and in a
statistically correct method. Compensation sliders can be selected to be displayed or hidden
on the density plot axes when the compensate box is checked. Compensation bars can also
be used to make compensation adjustments, however proper gating and match medians
must be used to compensate correctly