Bio-Rad S3™ Cell Sorter User Manual
Page 124
114 | S3 and S3e Cell Sorters
Troubleshooting
Table 30. Troubleshooting table (continued).
Error
Possible Causes
Troubleshooting Steps
Decontamination procedure
Software error
Contact technical support for help on the phone.
freezes during priming
Stream is spraying everywhere No tip in place
Run nozzle tip swap wizard and install nozzle.
No O-ring in place
Run nozzle tip swap wizard to reseat nozzle
with O-ring.
Tip is still clogged
Run nozzle tip swap wizard to clean nozzle using
sonication and syringe wash. Reseat nozzle.
Stream is spraying at an
Tip was not seated straight
Run nozzle tip swap wizard to clean nozzle and
angle with spray.
then reseat nozzle correctly.
No O-ring in place
Run nozzle tip swap wizard to reseat nozzle
with O-ring.
Wagon wheel has fallen out,
Run nozzle tip swap wizard to check if wagon
extra wagon wheel installed,
wheel is installed properly. Reseat nozzle.
or wagon wheel installed
incorrectly.
Tip is still clogged
Run nozzle tip swap wizard to clean nozzle using
sonication and syringe wash. Reseat nozzle.
Stream is moving around,
No O-ring in place
Run nozzle tip swap wizard to reseat nozzle
droplet camera shows
with O-ring.
droplets are moving
Wagon wheel has fallen out,
Run nozzle tip swap wizard to check if wagon
inconsistently
extra wagon wheel installed,
wheel is installed properly. Reseat nozzle.
or wagon wheel installed
incorrectly.
Tip is still clogged
Run nozzle tip swap wizard to clean nozzle using
sonication and syringe wash. Reseat nozzle.
Stream is hitting the back
Tip was not seated straight
Run nozzle tip swap wizard to clean nozzle and then
wall, deflection plates or
reseat nozzle correctly.
sort tray
No O-ring in place
Run nozzle tip swap wizard to reseat nozzle
with O-ring.
Wagon wheel has fallen out,
Run nozzle tip swap wizard to check check if wagon
extra wagon wheel, or wagon
wheel is installed properly. Reseat nozzle.
wheel installed incorrectly.
Tip is still clogged
Run nozzle tip swap wizard to clean nozzle using
sonication and syringe wash. Reseat nozzle.