Bio-Rad S3™ Cell Sorter - Manual (Page 59)

Instruction Manual | 49
4.5 Protocols and Workspace
A region can be moved, tilted, resized, and deleted (Figure 52).
1. Click the desired region.
2. To move region, hover till the crosshairs icon appears. Click and drag to new location.
3. To tilt, click the green circle indicator above the region and drag to change the angle.
4. To resize, click the circle or square indicators around the region and drag to the
appropriate size.
5. To delete, right click within the region and choose Remove region.
4.5.14 Creating Gates
Gates can be used between plots once
regions have been created. The purpose of
gating is to limit the events shown in one plot
by a region in another plot.
To create a gate (Figure 53):
1. Select the plot to be gated.
2. Right click within the plot.
3. Select Apply Gate to the region or
regions of interest.
The regions on the gated plot will appear in
the title bar of the plot. To ungate a plot, right
click in the gated plot and select Clear Gate.
Fig. 52. Moving or tilting a region within a plot.
Fig. 53. Density plot with Apply Gate selected.