Bio-Rad S1000™ Thermal Cycler - Manual (Page 24)

S1000 Thermal Cycler Manual
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Figure 12. The graphical screen is displayed.
2. When the S1000 thermal cycler completes running the protocol, the PROTOCOL
COMPLETE screen is displayed. After viewing this screen, press ENTER to return to the
main menu.
Canceling a Run
To cancel a running protocol:
1. Press CANCEL, then press the arrow key to select YES or NO.
2. Press ENTER to confirm the selection and continue to the next screen.
TIP: To cancel a protocol that is running on a dual 48/48-well reaction module,
press CANCEL and then select the block that you want to cancel the run on. Press
ENTER to confirm the protocol cancellation.
3. The PROTOCOL CANCELLED screen appears. This screen displays the total time that
the protocol ran before being cancelled.
4. Press ENTER again to return to the main menu.
Incubating Samples
Samples may be kept at a constant temperature for any amount of time. The incubation
continues indefinitely unless cancelled.
WARNING! Incubating samples for an extended period of time at 4–10°C,
particularly in areas of high humidity, can cause excessive moisture condensation
around the block.
To start incubating samples:
1. Load your samples into the thermal cycler block and press INCUBATE.
NOTE: For the dual 48/48-well reaction module, the block that contains the
samples must be selected before continuing to the next screen.
2. Select YES to use the heated lid during the incubation or NO to turn off the lid during
incubation. Press ENTER to confirm.
3. If using the heated lid, enter the lid temperature. Press ENTER to accept the default lid
temperature, or use the numeric keys to type a new lid temperature.
NOTE: Press PAUSE (.) key to enter a decimal point. To delete a number, press
CANCEL.
Flashing thick
bar shows
current step
Hold time for current step (counts up)
Protocol steps
(by temperature)
Target temperature
for the current step