Bio-Rad S1000™ Thermal Cycler - Manual (Page 13)

S1000 Thermal Cycler Manual
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4. Turn on the thermal cycler using the power switch on the back panel of the thermal
cycler base.
NOTE: Before operating the thermal cycler, be sure to read the safety
specifications (Safety and Regulatory Compliance on page ii) and operating
requirements.
5. When the S1000 thermal cycler starts up, it goes through two screens: the black booting
and the self-test screens. Once the self-test is run to verify proper functions, the main
menu is displayed. Use the main menu to begin operating the thermal cycler.
To remove the reaction module from the thermal cycler base, follow these instructions:
1. Turn off the thermal cycler.
2. Unlock and release the reaction module by pushing the locking bar down.
3. Carefully lift the reaction module out of the bay.
WARNING! Cooling fins may be hot immediately after running a protocol or
incubation. Before lifting the reaction module, make sure that the cooling fins are
not hot.
4. After removing the reaction module from the S1000 thermal cycler, store it on a clean,
flat surface where it cannot get bumped, scraped, or dropped.
Operating the Reaction Module Lid
The inner lid of the reaction module applies heat and force to the reaction vessel lids (caps or
tape) to produce consistent and successful reactions. Heating the inner lid prevents
condensation, while applying force seals the reaction to prevent evaporation.
WARNING! After a run, the heated inner lid can remain hot. Use caution when
opening and closing the lid.
To open the lid, use the following steps:
1. Turn the lid force knob counterclockwise to release the inner lid.
2. To open the lid, lift the lid lever up.
3. Lift the lid lever completely until the reaction module stays open without assistance.
To close the lid, use the following steps:
1. Push the lid lever down, making sure that the front of the lid is secured beneath the
housing.
2. Adjust the lid force by turning the lid force knob.
• Turn the knob clockwise to increase the lid force
• Turn the knob counterclockwise to decrease the lid force
3. Increase the lid force until the heated lid touches the reaction vessel. Continue to
increase the lid force based on the following recommendations:
• If using tubes, increase the lid force by a quarter turn after the lid touches the
tubes
• If using plates, increase the lid force by a half turn after the lid touches the plate
NOTE: The position marks on the lid indicate quarter turns.
WARNING! If the lid is tightened past the recommended force, tightening the lid
further will result in the knob slipping and causing a clicking sound. This is
designed as a warning that the lid force is past the recommended setting and may