Bio-Rad S1000™ Thermal Cycler - Manual (Page 21)

S1000 Thermal Cycler Manual
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8. Press ENTER to finish changing parameters in the step and to continue editing the
protocol.
Sample Volume and Lid Temperature
The sample volume and lid temperature influence the outcome of a PCR protocol.
• Sample volume — determines the temperature control mode, which influences the
amount of time the samples are held at the target temperature
• Lid temperature settings — determine the temperature of the heated lid and when it
cuts off. If the temperature is too high, the sample temperature might rise above the
target temperature
Temperature Control Modes
The S1000 thermal cycler uses one of two temperature control modes to determine when the
sample reaches the target temperature:
• Calculated mode — The thermal cycler calculates the sample temperature based
on the sample volume when a sample volume between 1 and 50 μl is entered for 96-
well or dual 48/48-well reaction modules, a volume between 1 and 30 μl is entered
for the 384-well reaction module, or a volume between 1 and 125 μl is entered for the
96-deep well reaction module. The calculated mode is recommended, because it
most accurately represents the actual sample temperature
• Block mode — When a sample volume of zero (0) μl is entered, the thermal cycler
assumes that the sample temperature is the same as the measured block
temperature