Bio-Rad S1000™ Thermal Cycler - Manual (Page 17)

S1000 Thermal Cycler Manual
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3. Using the arrow keys, select TEMP to enter a temperature step or GRAD to enter a
gradient temperature step in the protocol file. Press ENTER to continue to the next
screen.
NOTE: The first step in a protocol must be either a TEMP or GRAD step.
4. Enter the target temperature between 0 (zero) and 100.0°C for the temperature step.
Press ENTER to continue to the next screen.
5. Enter the hold time (TIME) in minutes and seconds using the numeric keys. The hold
time (TIME) ranges between 0:01 (one second) and 18:00:00 (18 hours). Entering 0 (zero)
adds an infinite hold and holds this step forever. Press ENTER to continue to the next
screen.
6. Select YES, NO, or OPTION by pressing the right and left arrow keys.
• YES — to confirm the current parameters for this protocol step
• NO — to change a parameter in this protocol step
• OPTION — to add more parameters to this protocol step. For more information
about entering options, see Adding an Option to a Temperature Step (page 10)
Press ENTER to continue to the next screen.
7. Enter the lower temperature in the gradient. The lower temperature is at the front (row H)
of the block.
8. Enter the upper temperature in the gradient. The upper temperature is at the back (row A)
of the block.
NOTE: The range of temperatures is limited by the widest available range for
gradient, which is 24°C. The highest value that can be entered for the upper
temperature is 100°C.
9. Enter a hold time between 0:01 (one second) and 18:00:00 (18 hours).
10.Select YES, NO, or OPTION by pressing the right and left arrow keys.
• YES — to confirm the current parameters for this protocol step
• NO — to change a parameter in this protocol step
• OPTION — to preview the temperature gradient. If Option is selected, select
VIEW on the next screen to view the gradient or EXT to add a hold time extension.
Press ENTER to continue to the next screen.
11.Repeat the instructions in steps 5–8 to continue entering additional temperature steps.
NOTE: A protocol can contain up to 99 protocol steps. The first step must be a
temperature (TEMP) step, while the last step must be an END step.
12.(Optional) To enter a GOTO step immediately after the set of steps to be repeated in a
cycle, use the arrow keys and select GOTO. Press ENTER to continue to the next
screen.