Eppendorf Multiporator - Manual (Page 36)

78
Step 2 – Starting cell fusion
– Trigger the cell
fusion process
by Pressing the
START key.
– "Charge" appears
briefly in the fourth
line of the display.
Charging
commences.
– During the charging
procedure,
alternating voltage
U'
~
is applied for the
selected duration.
The alternating
voltage is
represented by the
symbol
~ (sine-wave
oscillation).
– Discharge occurs
after the pre-
counted alignment
time (0–95
seconds). This is
indicated by a flash
in the fourth line of
the display.
If the number n
which has been
entered is n >1,
a further discharge
occurs each time
after one second.
12.AUG.00 13:42
U'
~
5V t 30s
U 30V t 15µs n2
Charge
12.AUG.00 13:42
0:23
U'
~
5V t 30s
U 30V t 15µs n2
5 Mode of operation
Multiporator_Text_en.fm Seite 78 Freitag, 9. Juni 2006 8:05 08