Eppendorf Multiporator - Manual (Page 29)

71
5.1 Mode for electroporation of eukaryotic cells
This mode is represented in the display by the symbol .
Instructions for the filling of the electroporation cuvettes have been
summarized in chapter 4.7.1 of the operating instructions. A detailed
description of the electroporation of eukaryotic cells can be found in the
Basic Applications Manual for electroporation, which is included in the
delivery package. This application manual can also be downloaded from
the Eppendorf Homepage www.eppendorf.com.
Step 1 – Entering / modifying parameters
Note: The course of the curve is calculated internally, so that the course
of the discharge curve (e-function) is maintained according to the
parameters which have been entered.
– If necessary, switch
to the mode
by
pressing the MODE
key.
– Press the SET key
to select the
parameter desired
(the parameter will
be underlined).
– Use the arrow keys
to modify the
parameter in the
direction required.
– If necessary, modify
other parameters
using the SET key
and the arrow keys.
– Terminate the
entering procedure.
The parameters
are effective
immediately and
do not have to be
stored.
Example of a display for
the electroporation of
eukaryotic cells.
The relevant parameters
appear in the display in
larger letters and in bold
print.
U: Voltage, in V (volt).
Can be set in
increments of 1 V in
the range 20–100 V;
in increments of 10 V
in the range
100–1,000 V;
in increments of
100 V in the range
1,000–1,200 V.
τ
: Time constant, in µs
(microseconds).
Can be set in
increments of 5 µs in
the range 15–500 µs.
n: Number of pulses
during the
experiment.
Can be set between
1 and 99. A period of
one minute elapses
between each pulse.
12.AUG.00 13:37
U 500V
τ
220µs
n 2
5 Mode of operation
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