4 device description – Eppendorf Multiporator User Manual

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When screwing the core, make sure that no air bubbles form.

Fig. 10: Filling the Helix fusion chamber (schematic representation)

Leave the closed Helix fusion chamber upside-down on the coaxial
connector until the fusion process has been completed!

4.8.6 Fusion and cell suspension extraction

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Slide the fusion insert ﬁrmly into the device.

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Place the closed Helix fusion chamber onto the coaxial connector
and lock it into place by rotating it a quarter-turn.

–

Carry out the fusion with the set parameters (see chap. 5.3).

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Loosen the Helix fusion chamber carefully by rotating it a quarter-turn
and then remove it.

–

To open the chamber, place it in a vertical position (e.g. in the stand).

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Unscrew the core carefully from the beaker.

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Using a small quantity of the appropriate medium, rinse off any cell
suspension that may be on the core.

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The cell suspension from the beaker is mixed with the cell suspension
that has been rinsed off and is then treated further as required.

4 Device description

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