Eppendorf Multiporator - Manual (Page 31)

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5.2 Mode for electroporation of bacteria and yeast (optional)
This mode is represented in the display by the symbol .
Instructions for the filling of the electroporation cuvettes have been
summarized in chapter 4.7.1 of the operating instructions. In addition,
application protocols for the electroporation of bacteria, yeasts and other
microorganisms can be downloaded from the Eppendorf Homepage
www.eppendorf.com.
Step 1 – Entering / modifying parameters
– If necessary, switch
to the mode
by
pressing the MODE
key.
– Press the SET key
to select the Voltage
(U) parameter (the
parameter will be
underlined).
– Use the arrow keys
to modify the
parameter in the
direction required.
– Terminate the
entering procedure.
The parameters
are effective
immediately and
do not have to be
stored.
Example of a display for
the electroporation of
bacteria and yeast.
The relevant parameters
appear in the display in
larger letters and in bold
print.
U: Voltage, in V (volt).
Can be set in
increments of 10 V
in the range
200–1,000 V;
in increments of
100 V in the range
1,000–2,500 V.
τ
: Time constant,
in ms (milliseconds).
Set to 5.0 ms.
12.AUG.00 13:42
U 2000V
τ
5 ms
5 Mode of operation
Multiporator_Text_en.fm Seite 73 Freitag, 9. Juni 2006 8:05 08