Eppendorf Multiporator - Manual (Page 19)

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– Connect the Micro fusion chamber with the coaxial connection of the
cable.
– Focus the electrodes and cells under the microscope.
– Run through the parameters by pressing the START key. This can be
monitored under the microscope (see chap. 5.3).
Fig. 6: Microscopic view of the cells during the alignment and after the fusion
sequence with individual fusion products
The parameters determined to be optimal can be directly adopted for the
fusion in the Helix fusion chamber (see chap. 4.8.4).
4.8.2 Micro fusion chamber (Electrode gap width 0.5 mm)
The non-standard accessories (see "Ordering information") contain a
Micro fusion chamber with a modified electrode interval of 500 µm. With
the help of this Micro fusion chamber, cells can be fused under
microscopic control.
General instructions for usage of a Micro fusion chamber are given in
chapter 4.8.1 and in the Basic Applications Manual for electrofusion. This
is included in the delivery package of the fusion module.
Important note: When calculating the fusion parameters (see Basic
Applications Manual for electrofusion), the modified electrode interval of
500 µm must be taken into account. Please note that the parameters
determined as optimal cannot be adopted directly for fusion in the Helix
fusion chamber.
4.8.3 Cleaning the Micro fusion chamber
The content of the Micro fusion chamber is rinsed out using bi-distilled
water from a spray bottle. Cell residue that is particularly stubborn can be
removed by carefully cleaning the electrodes with a soft toothbrush using
vertical strokes. (The space between the electrodes must not be changed
during this process!) The drying process can be accelerated by rinsing the
chamber with 70 % non-denatured ethanol.
200 µm
Cells after fusion and fusion products Alignment of the cells
between the electrodes
4 Device description
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