Eppendorf Multiporator - Manual (Page 20)

62
4.8.4 Helix fusion chamber
The Helix fusion chamber has been specially designed for the production
of larger quantities of fusion products (hybrids). It consists of a conical
tapered core that carries the electrodes and a beaker into which the cell
suspension is added (see fig. 7).
Fig. 7: Helix fusion chamber
Diagram of Helix fusion chamber
The Helix fusion chamber (see fig. 8) consists of a cup (1) and a core with
parallel wound platinum wires, which are the electrodes (2). They are
wound around the core as a double helix. Both parts are screwed
together. The gap (4) between the cup and the core has a filling volume of
250 µl.
The Helix fusion chamber is linked to the insert (6) for cell fusion via the
coaxial connector (3).
Fig. 8: Schematic representation of the Helix fusion chamber and the fusion insert
Cup Core Helix fusion chamber Fusion insert
1 Thread 2 Electrodes
3 Coaxial 4 Gap for cell fusion 5 Contacts
6 Coaxial connector
3
1
2
4
5
6
connector
4 Device description
Multiporator_Text_en.fm Seite 62 Freitag, 9. Juni 2006 8:05 08