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BUCHI Rotavapo R-220 SE User Manual

Page 48

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5 Putting into operation

48

R-220 SE Operation Manual, Version F

The following concentrations per germ were applied for every contaminant:
10

3

germs/ml

10

4

germs/ml

10

5

germs/ml

10

6

germs/ml

5 .11 .1

Controlled contamination and distillation

5 l of sterile distilled water were filled into the evaporating flask, contaminant was added to reach the
corresponding concentrations per germ and 1 l was distilled off under the following conditions:
Heating bath set to 60 °C, vacuum set to 42 mbar (corresponds to a boiling point of water of 30 °C),
chiller set to 7 °C.
Afterwards a sample of the distillate was taken (

Sample 1 in the table) by means of a sterile pipette

and bottle for determination of germ count.

5 .11 .2 Disinfection

The evaporating and the receiving flask were emptied and the evaporating flask was rinsed with
water.
1.5 l of isopropanol were filled into the evaporating flask and the evaporation was started under the
following conditions:
Heating bath set to 60 °C, vacuum set to 250 mbar (corresponds to a boiling point of isopropanol of
50 °C), chiller set to 7 °C.
At first, the chiller was switched off until the vapor passed the whole instrument. Then the chiller was
switched on again and 0.75 l were distilled off. Afterwards the evaporting and the receiving flask were
emptied and the instrument was dried at a vacuum of < 20 mbar.

5 .11 .3

Sampling for determination of the germ count

The evaporating flask was filled with 2 l of sterile distilled water and 0.75 l were distilled off under the
following conditions:
Heating bath set to 60 °C, vacuum set to 42 mbar (corresponds to a boiling point of water of 30 °C),
chiller set to 7 °C.
A sample of the distillate (

Sample 2 in the table) and of the residue within the evaporating flask

(

Sample 3 in the table) was taken for the determination of the germ count.

5 .11 .4

Final cleaning and drying

The evaporating and the receiving flask were emptied and the evaporating flask was rinsed with
water.
1.5 l of isopropanol were filled into the evaporating flask and the distillation was started under the
following conditions:
Heating bath set to 60 °C, vacuum set to 250 mbar (corresponds to a boiling point of isopropanol of
50 °C), chiller set to 7 °C.
At first, the chiller was switched off until the vapor passed the whole instrument. Then the chiller was
switched on again and 0.75 l were distilled off. Afterwards the evaporting and the receiving flask were
emptied and the instrument was dried at a vacuum of < 20 mbar.