Bio-Rad Microplate Manager Software - Manual (Page 90)

Appendix
82
Percent Control Assay Tutorial
1 Defining the Template
2 Setting up the Equations
3 Setting up the Screening Report
4 Entering Experimental Info
5 Setting up the Report
An example of how to set up an ELISA Assay that uses a control and equations to
transform the absorbance data into percentage values of the control well (CNTRL) is
given here. This assay has no standards and no standard curve.
Open sample data file (Percent Control.mpl) with its protocol and template.
C:\Program Files\Bio-Rad\Microplate Manager 6\Sample Data
1 Defining the Template
1. Click on the Template icon in the Main Toolbar
• Blanks are defined as #. The average of these # wells will be subtracted
from all the wells
• BLK are a second reagent blank. The average of these BLK wells will be
subtracted from all the wells.