Dilution data assay tutorial, 1 defining the template, 1defining the template – Bio-Rad Microplate Manager Software User Manual
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Appendix
68
Dilution Data Assay Tutorial
1.
Defining the Template
2.
Setting up the Dilutions
3.
Selecting Curve Fit and Customizing Curve Fit Plot
4.
Entering Experimental Info
5.
Setting up the Report
An example of how to set up an ELISA Assay including a dilutions and a standard
curve is given here. The Report is customized to show interpolated concentration,
dilution factor, conc X dil factor, and mean concentration for the samples.
Open sample data file (Dilution Data.mpl) with its protocol and template
C:\Program Files\Bio-Rad\Microplate Manager 6\Sample
Data
1
Defining the Template
1. Click on the Template icon in the Main Toolbar
2. Type 200 in well A1 and press Enter
3. Highlight area well A1-G2. (Click & hold mouse on well A1, drag
mouse to well G2, and release.)
4. Click on the Fill Series icon in the Template Toolbar