Bio-Rad Microplate Manager Software - Manual (Page 73)

Appendix
65
Quantitative ELISA Assay Tutorial
A tutorial showing how to set up an ELISA Assay, including a standard curve and
equations to transform the absorbance data into percentage values of the maximum
bound well, is given here.
1. To set up the protocol, Choose File > Instrument Setup.
2. Select Endpoint Reading Mode and the desired wavelength.
3. Open sample data file (Percent Max Binding Assay) with its protocol &
template.
C:\Program Files\Bio-Rad\Microplate Manager 6\Sample
Data
Defining the Template
Click the Template icon in the Main
Toolbar.
Blanks are defined as #. The average of
these # wells will be subtracted from all
the wells.
NSB are non-specific binding wells.
MAX is for the maximum binding well.