Bio-Rad Microplate Manager Software - Manual (Page 19)

Quick Start
11
Open a saved protocol or set up a new protocol (by setting up a template, and
selecting the instrument, assay info, analysis options and print options). Refer to
the
Instrument Controls
1. Click File > Read New Plate. The Instrument Controls dialog displays.
2. Under the Reader tab, set the Reading mode: Endpoint, Kinetic, or
Plate 2-1.
If you are using an xMark, Spectra is another Reading mode option.
3. Select the other Reading options:
• Read speed (iMark only)
• Wavelength (nm) and single / dual
• Mix time & speed, and mix type (xMark only)
• Pathlength Correction
1
1 Pathlength correction is used to calculate the OD (or % transmission)
for each well, normalized to 1 cm fluid depth (d) in each well.