Bio-Rad Microplate Manager Software - Manual (Page 55)

Analyzing Data Using Cutoffs
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Reference can be made to standard or sample IDs that have been defined in the
Template setup
• Standards: enter ! followed by concentration
• Unknowns: enter identifier
• Blanks: enter
#
In all Cutoff equations, Microplate Manager 6.0 uses the averaged value of that
group.
Examples of valid expressions:
• U01
• (U01+.1)/2
• (!100-!10)*2
Cutoff Examples for Assays with Controls
Positive & Negative Controls – positive (POS) & negative (NEG) control well(s)
defined on template
• Low Cutoff: NEG
• High Cutoff: POS
% B/Bo – non-specific binding blank (NSB) & maximum or Bo well(s) (MAX)
defined on template
• Low Cutoff: NSB
• High Cutoff: MAX
% Calibrator –blank (#) & calibrator (CALB) well(s) defined on template
• Low Cutoff: #
• High Cutoff: CALB
% Control - non-specific binding blank (NSB) & control well(s) (CNTRL) defined
on template
• Low Cutoff: NSB
• High Cutoff: CNTRL