Bio-Rad PharosFX™ and PharosFX Plus Systems - Manual (Page 9)

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The PharosFX (Plus) imaging system consists of:
• Laser scanner
• Sample Tray set, including: glass sample tray, transilluminating screen, and sample
holders
• Pre-installed filters for fluorescence detection: 640BP, 605BP, blank filter holder for
custom filters. PharosFX Plus has an additional 390BP filter for phosphor screens
• External Laser Module is optional
(1) Laser Scanner
The PharosFX and PharosFX Plus have an internal laser that emits light at 532 nm only. All
PharosFX units can scan at resolutions of 50, 100, 200, and 800 microns and have a linear
dynamic range that extends over 4.8 orders of magnitude (1:65536). In contrast, x-ray film
has a linear dynamic range that is limited to only 1.5 orders. The scanner also contains fully
automated emission filter wheels with 6 filters. These combined features permit the
PharosFX to image almost any fluorescent dye and to scan multi-color fluorescence
applications, in addition to storage phosphor imaging with the PharosFX Plus.
(2) Sample Tray
The glass Sample Tray is used as a scanning platform for fluorescent gels or blots and
K-type phosphor screens. Its spill resistant design exhibits very low background fluorescence
and high chemical resistance.
Additional Multi-Sample trays can be purchased to accommodate thick agarose gels, gels
within glass plates (1707819 Multi-Sample Tray II), metal backed storage phosphor
screens,and microtiter plates (1707812 Multi-Sample Tray).
Fig. 2.2. a) Multi-Sample Tray, b) Multi-Sample Tray II
(3) Optional External Laser Module
The optional external laser module provides one or more additional excitation source(s).
The current configurations for lasers are:
a) 488 nm Only
b) 488 nm and 635 nm