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Bio-Rad PharosFX™ and PharosFX Plus Systems User Manual

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Fig. 4.1. PharosFX LCD and control panel.

An LCD "Contrast" button is located on the control panel (Figure 4.1). This button can be
used to adjust the brightness of the LCD display. The contrast function cycles so that
holding the button down will cause the display to get lighter and then darker again.

The "On Line" button on the control panel should be depressed only in the unlikely event
that the acquisition software cannot halt scanner operation.

4.2 Fluorescence Detection

4.2.1 Sample Preparation

Prepare fluorescent samples as recommended by published protocols. The following sample
types can be imaged using PharosFX and PharosFX Plus.

Agarose Gel

Acrylamide Gel

Acrylamide Gel sandwiched between two glass plates

Acrylamide Gel on top of a single glass plate

Membranes and Blots

Hybridized Glass Plates

Microtiter Plates