Bio-Rad PharosFX™ and PharosFX Plus Systems - Manual (Page 22)

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Table 4.1
Sample Preparation and Accessories
Caution: When using the glass sample tray the maximum sample thickness must not
exceed 8 mm. The sample tray cannot be inserted into the scanner with a sample thicker
than 8 mm. The height of the side rail on the tray can be used as a guide to determine the
maximum sample thickness, as the sample's height must not exceed that of the side rail.
Thicker samples, up to 17 mm, can be imaged using the Multi-Sample Tray I (1707812) or
Multi-Sample Tray II (1707819).
4.2.2 Inserting a Fluorescent Sample into the Scanner
1. Open the front door of the scanner.(Figure 4.2)
2. Pull out the glass sample tray and, if dirty, clean with a dry lint-free cloth.
3. Carefully place the prepared gels or blots onto the tray, making sure that the fluorescent
signal is directed upwards. (Maximum sample thickness is 8mm.)
4. Note the coordinates of the sample on the tray for reference when selecting the desired
scan area in the software. The grids with coordinates on the tray in the software match.
Accessory Use for Preparation Notes
Sample Tray
170-7811 • Agarose Gels
• Acrylamide Gels
• Blots/Membranes
• Colorimetric Gel
Documentation
• Unmounted Storage
Phosphor Screens
• No gels thicker than 8 mm.
• Gels should be wet.
• Blots/Membranes should be moist.
• Use Sample Holders (170-7813) to
keep sample from moving during scan.
• When performing Colorimetric Gel
Documentation work, utilize the
transilluminating screen supplied.
• Will accept unmounted screens from
many manufacturers including Bio-
Rad, Kodak, MD and Fuji.
• When working with 20 x 25 cm small
format screens (170-7843) utilize the
alignment overlay supplied with the
Sample Tray.
Multi-Sample Tray
170-7812
• Mounted Screens (MD
format)
• Microtiter Plates
• Face MD screens upward inside the
tray.
• When scanning Microtiter Plates use
the Microtiter Adaptor Assembly (170-
7814).
• Microtiter Adaptor Assembly accepts
up to 8 microtiter plates.
• Plates that can be scanned include 96,
384 and 1536 wells.
• Make sure prior to preparing samples
that the microtiter plate format can fit
inside the scanner when used with the
Multi-Sample Tray/Microtiter Adaptor
Assembly.
Multi-Sample Tray II
170-7819
• Acrylamide gels
sandwiched between
glass plates.
• Acrylamide gels sitting
on glass with no top
glass plate.
•
• Make certain that the thickness of the
sample and the glass plates fits within
the scanner prior to scanning.
• The Multi
-
Sample Tray II ships with
three sets of non
-
slip spacing strips.
Use these to determine your optimal
focus for Differential Display
workscanning.
• Utilize the Print 1:1 function to aid in
any incision work for Differential
Display processes.
TLC plates
!